PathoSEEK® Bile Tolerant Gram Negative Detection Assay v2 (200 rxns)

Note: This product has been upgraded to the Bile Tolerant Gram Negative Detection Assay v3 Kit (Item #420539), offering improved performance and reliability. We’ll continue to fully support Item #420149 through July 2026 to ensure a smooth transition. View the v3 Kit here.

The PathoSEEK® The Bile Tolerant Gram Negative (BTGN) Detection Assay v2 is used in combination with SenSATIVAx® DNA Purification Kits and qPCR Master Kit v3 to detect species of bacteria that can survive in the human stomach and are potentially harmful to humans in cannabis and hemp flower, concentrates, or infused products.

PathoSEEK Detection Assays are multiplex tests based on gene amplification and detection by quantitative PCR. Each PathoSEEK Detection Assay contains primers and probes specific for the target species as well as an internal cannabis control. The target organisms will be read on the following fluorophores:

• Cannabis DNA - HEX
• BTGN - FAM

PathoSEEK® Bile Tolerant Gram Negative (BTGN) Detection Assay is approved for use on either the Agilent AriaMx Real-Time PCR instrument or the BioRad CFX96, and it must be used in combination with:

• SenSATIVAx® DNA Purification Kit
• qPCR Master Kit v3
• BTGN / Coliform / Entero Positive Control