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PathoSEEK® Listeria Detection Assay

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$379.05
SKU: 420185
Quantity: 200 reactions
Storage Conditions: -25ºC to -15ºC

ABOUT THIS PRODUCT:

Note: This product has been upgraded to the Listeria Monocytogenes Detection Assay v2 Kit (Item #420527), offering improved performance and reliability. We’ll continue to fully support Item #420185 through July 2026 to ensure a smooth transition. View the v2 Kit here.

The PathoSEEK® Listeria Detection Assay is used in combination with SenSATIVAx® DNA Purification Kits and qPCR Master Kit v3 to detect Listeria monocytogenes

PathoSEEK Detection Assays are multiplex tests based on gene amplification and detection by quantitative PCR. Each PathoSEEK Detection Assay contains primers and probes specific for the target species as well as an internal DNA control. The target organisms will be read on the following fluorophores:

  • Internal DNA Control - HEX
  • Listeria - FAM

PathoSEEK® Listeria Detection Assay is approved for use on the Agilent AriaMx Real-Time PCR instrument and the BioRad CFX96, and it must be used in combination with:

  • SenSATIVAx® DNA Purification Kit 
  • qPCR Master Kit v3
  • PathoSEEK® Listeria Positive Control
  • Listeria Enrichment Broth


Note: Listeria cells are slow-growing and can be rapidly out-grown by competitors. This is why we require samples be incubated for 24-48 hours at 35C in Listeria Enrichment Broth for selective enrichment.

Details

All Medicinal Genomics reagents are manufactured in a facility certified to ISO 9001 standards. 

All lots of reagents are functionally tested in-house, and Certificates of Analysis (COAs) are available upon request.

Please contact support@medicinalgenomics.com if you have any questions or concerns. 

FEATURES

Fast and scalable workflow

  • Same-day results 
  • Turnkey automation is available with leading liquid handling systems
  • Multiplex assays maximize instrument capacity 

Validated for Use on Multiple Matrices

  • Cannabis/Hemp flower
  • Oil 
  • Chocolate 
  • Candies 
  • Stainless steel
  • And more! 

Advantages Over Culture Methods 

  • Results in hours, not days! 
  • Better reproducibility of results 
  • Easily automated with liquid handling robotics 

DISCLAIMER

This test was developed, and its performance characteristics determined by Medicinal Genomics Company, for laboratory use. Any deviations from this protocol are not supported by MGC.

This test has not been validated on remediated (irradiated, ozone treated, acid treated, hydrogen peroxide treated, etc.) samples. Samples that have undergone remediation may cause discordant results between plating methods and PathoSEEK methods. When remediated samples produce a result above the action limit on qPCR, we recommend confirming viability with an approved plating method.

Results may vary based on laboratory conditions. Altitude and humidity are factors known to affect the growth of bacterial and fungal species.

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