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PsiloSEEK® P. Cubensis Speciation Assay (200 rxn)

SKU: 419001
Storage Conditions: -20C


The PsiloSEEK® P. cubensis Speciation Assay is used with SenSATIVAx® MIP DNA Extraction Kit (Mushroom Tissue) or Leaf Punch Lysis Solution (Mushroom Spores) and qPCR Master Kit v3 to purify DNA from mushroom tissue and spore samples and detect Psilocybe cubensis ITS and Wood Lovers ITS in a single qPCR (Quantitative Polymerase Chain Reaction).

P. cubensis can be easily confused for other mushrooms in the Psilocybe genus, some of which can be toxic to humans. In particular, the Wood Lovers Psilocybe class causes acute paralysis upon ingestion known as Wood Lovers Paralysis (WLP).

The targets will be read on the following fluorophores:

  • P. cubensis ITS - FAM
  • Wood Lovers ITS - CY5 channel
  • PsiloSEEK Spike-in Control - ROX

The PsiloSEEK P. cubensis Speciation Assay is approved for use on either the Agilent AriaMx Real-Time PCR instrument or the BioRad CFX96, and it must be used in combination with:

  • SenSATIVAx® MIP DNA Extraction Kit (Mushroom Tissue) or Leaf Punch Lysis Solution (Mushroom Spores)
  • qPCR Master Kit v3
  • PsiloSEEK Positive Control
  • PsiloSEEK Spike-In Control



Unmatched Expertise in Psychedelic Mushroom Genomics
Our team published the most comprehensive, chromosome scale annotated reference genome for Psilocybe cubensis, and since then we have sequenced more than 100 P. cubensis genomes. We used that information and experience to design the PsiloSEEK assay so that labs and growers can confirm P. cubensis genetics with a simple qPCR assay. 

A Proven Record in qPCR  
Our team has provided Cannabis-specific qPCR assays to the field since 2015, covering a variety of targets, including human pathogens, plant pathogens, plant sex, genetic trait screening, and more. Testing labs around the world trust Medicinal Genomics, and several assays have received AOAC approval. 

Designed for Flexibility and Scalability  
PsiloSEEK can be run on multiple qPCR instruments and easily automated on liquid handling automation solutions from Agilent Technologies, Hamilton Robotics, and MGI/BGI. 

All Medicinal Genomics reagents are manufactured in a facility certified to ISO 9001 standards. All lots of reagents are functionally tested in-house and Certificates of Analysis (COAs) are available upon request.
Please contact support@medicinalgenomics.com if you have any questions or concerns.