The PathoSEEK® Tobacco Mosaic Virus Detection Assay is a reverse-transcriptase quantitative PCR (RT-qPCR or QRT-PCR) assay that screens for the presence of Tobacco Mosaic Virus.
Tobacco mosaic virus (TMV) is an extensively studied RNA virus known to infect tobacco (Nicotiana tabacum) and other solanaceous crops.
It causes splotchy or twisted leaves, strange mottling symptoms (a “mosaic”), slowed growth, and reduced yields. Mosaic virus has spread to several other species of plants besides tobacco, including cannabis plants.
Note: TMV doesn’t affect humans, but plants that get infected by mosaic virus may not grow as fast or yield as well as they could have.
Like all PathoSEEK® assays, cannabis control is multiplexed in and utilizes the HEX optical channel.
This product is used with our Leaf Punch Lysis Buffer and our RT-qPCR Master Kit.
This product requires the following optical channels on your qPCR instrument.
- Tobacco Mosaic Virus is on the FAM channel
- Cannabis Internal Control is on the HEX channel
