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PathoSEEK® 2-Color Aspergillus Multiplex (200rxn)

SKU: 420130
Storage Conditions: -20C


The Aspergillus Multiplex Detection Assay allows laboratories to test cannabis samples for four pathogenic species of Aspergillus (A. fumigatus, A. flavus, A. niger, and A. terreus) in a single well.

This not only reduces time and labor for cannabis testing laboratories, it also frees up additional wells so labs can process more samples on each instrument run. For example, California labs can test one cannabis sample for all six required microbial targets, using just two wells! The Aspergillus Multiplex in one well and the STEC/Salmonella Multiplex in the other.

We designed the Aspergillus Multiplex assay so that all four species are on the same channel (FAM). That means detection of any of the four pathogenic Aspergillus species will trigger a curve on the FAM channel. We will continue to offer individual Aspergillus assays in our webstore, so labs can run a subsequent test to determine which species caused the positive result.

** UPDATE ** We are proud to announce the launch of a new Aspergillus Multiplex Assay in 5 Colors (5 Channels) enabling full speciation in a single well. If reruns are required or are part of your workflow for positive Aspergillus hits, then check out the newest addition to the PathoSEEK® platform menu. The Aspergillus 5 Color Multiplex Detection Assay is the only multiplex assay on the market able speciate 4 species and an internal control on 5 channels in one reaction.


Specifically designed for use across the cannabis and hemp matrices, including flower, concentrates, and a range of infused products, the PathoSEEK platform uses qPCR technology, which has been proven in the food and healthcare industries, to be the most accurate, repeatable, and automatable product safety testing methodology.

All Medicinal Genomics reagents are manufactured in a facility certified to ISO 9001 standards. All lots of reagents are functionally tested in house and Certificates of Analysis (COAs) are available upon request.

Please contact support@medicinalgenomics.com if you have any questions or concerns.


AOAC Certified
You can use the PathoSEEK 5-Color Aspergillus Multiplex assays with confidence because they were specifically designed for use on cannabis matrices and the AOAC PTM has certified its use for:

  • Next-day results. 24-hour enrichment for cannabis flower
  • Multiple matrices. Cannabis flower, infused edibles, and concentrates

View the AOAC Certificate

No Re-runs Necessary
Previous Aspergillus multiplex assays required labs to run four species-specific assays to identify which type of Aspergillus triggered a failure. The new PathoSEEK® 5-Color Aspergillus Multiplex Assay does it all in a single qPCR reaction. This means your lab can stock fewer reagents, deliver faster results, and save money!

Cannabis DNA Internal Control
All PathoSEEK qPCR assay internal controls target a sequence in the cannabis genome, which helps to ensure plant cells have been lysed to release pathogens growing endophytically, DNA survived extraction intact, and there is no contamination in your Assay Master Mix.

Primers and Probes Designed for Cannabis
All PathoSEEK primers and probes were tested in-silico against the cannabis genome to ensure no cross-reactivity. Other platforms that use primers and probes borrowed from food testing may inadvertently hit the cannabis genome.

Universal Cycle Time
All PathoSEEK assays use the same cycle program, so you can run several different assays simultaneously.

Turnkey Automation
DNA purification and qPCR setup can be fully automated with turnkey solutions from Agilent Technologies, Hamilton Robotics, and MGI/BGI.


This test was developed, and its performance characteristics determined by Medicinal Genomics Company, for laboratory use. Any deviations from this protocol are not supported by MGC.

This test has not been validated on remediated (irradiated, ozone treated, acid treated, hydrogen peroxide treated, etc.) samples. Samples that have undergone remediation may cause discordant results between plating methods and PathoSEEK methods. When remediated samples produce a result above the action limit on qPCR, we recommend confirming viability with an approved plating method.

Results may vary based on laboratory conditions. Altitude and humidity are factors known to affect the growth of bacterial and fungal species.