The PathoSEEK® Salmonella & E. coli Multiplex Assay is used to detect a wide variety of species classified as salmonella and E. coli. The Assay targets E. coli species using the FAM fluorophore and Salmonella species using the ROX fluorophore. It is used in combination with the SenSATIVAx Extraction Kit and qPCR Master Kit. It is also recommended to use the Salmonella & E. coli Multiplex positive control.
Note: This product was recently updated in April of 2019 to include the ROX fluorophore instead of the Texas Red fluorophore for Salmonella spp.
This version 2 assay was updated in May of 2020 from our original version 1 assay to include additional species. To remain backwards compatible with our historical primer sets in version 1, we have opted to add additional primers to our mastermix as opposed to altering the historical primers.
For more information please visit: https://help.medicinalgenomics.com/salmonella_v2_assays_whats_new
All Medicinal Genomics reagents are manufactured in a facility certified to ISO 9001 standards. All lots of reagents are functionally tested in house and Certificates of Analysis (COAs) are available upon request. Please contact firstname.lastname@example.org if you have any questions or concerns.