The PathoSEEK® Total Yeast and Mold Detection Assay is used to detect species classified as yeasts and molds. The results of a Total Yeast and Mold (TYM) test are an indication of yeast and mold contamination on a cannabis sample. The Assay targets these species using the FAM fluorophore, and is used in combination with MGC SenSATIVAx and qPCR Master Kits. It is also recommended to use the Total Yeast and Mold positive control.
Regulations for total count microbial threshold tests currently use nomenclature from culture-based methods to set acceptable thresholds. It is necessary to convert the quantification cycle (Cq) value from the qPCR assay to colony forming units (CFU). In order to maintain the best possible concordance with plating, the PathoSEEK® Total Yeast and Mold Assay does not include certain species that fail to culture. Powdery Mildew and Botrytis are two such species and are therefore omitted in the assay design.
For more detailed information about our assays and a complete list of qualitative inclusion and exclusion data for our primers, please visit our help center or see the following relevant articles.
All Medicinal Genomics chemicals and reagents are manufactured in a facility certified to ISO 9001 standards