Tryptic Soy Broth is a growth medium that is used to homogenize the sample before the DNA extraction step. It is also used for overnight incubation in our microbial detection assay protocol. This product is not intended to be used for the diagnosis of human disease.

Tryptic Soy Broth, also known as Soybean-Casein Digest, conforms to the formula given by the U.S. Pharmacopeia. This medium contains digests of soybean meal and casein, which provide amino acids and other nitrogenous substances, making it a highly nutritious medium for a variety of organisms. It is commonly recommended for use as a general purpose medium for the isolation and cultivation of a wide variety of bacteria and fungi.

Limitations and Quality Statement

Due the manufacturing process and ingredients of culture medias, TSB will sometimes contain non-viable organisms that will be evident on a gram stain. 

Culture media should not be used if it shows signs of microbial contamination, dehydration (shrinking or cracking of the medium), hemolysis, discoloration, or is beyond the expiration date.